In her practice, Paky Vlassopoulou explores issues such as the production of knowledge, historization and ruins. Most recently, she has been particularly interested in the politics of service industries in relation to notions of care and hospitality. Her work often takes the form of an event, open to participation and discussion. In 2012, together with artists Chrysanthi Koumianaki and Kosmas Nikolaou, she co-founded 3 137, an artist run space operating in Athens, Greece.

Paky Vlassopoulou has had work commissioned by NEON Foundation and Whitechapel Gallery at the Gennadius Library/The American School of Classical Studies, Athens; and has exhibited work in a project by the New Museum, New York, and the DESTE Foundation, at the Benaki Museum, Athens, and at the School of the Museum of Fine Arts, Boston, among others.